RECONSTITUTING PEPTDES 101:
‘Don’t Destroy Your Research Before It’s Even Begun’
ALL OUR PEPTIDES:
Synthesized within 28 days or less of purchase
When it comes to Research products, peptides are actually the best products to research with, showing consist results time and time again. Peptides help build structure, catalyze reactions, regulate and protect and transport substances throughout the body. From lipolysis, anti-aging, wound repair, artery & vein regeneration, increase in memory function to killing bacteria like MRSA and Candida. The potential for these peptides are only scratching the surface, they are virtually limitless. Even medical practitioners are now starting to utilize them.
However, there is one main component surrounding peptides that is being done improperly, leaving most researchers with subpar results at best, to what could have been a break-through finding. What is that integral step that researchers need to master to get the full effects out of these amazing peptides…?
PROPER PEPTIDE RECONSTITUTION
I know you have heard that peptides are fragile, and they are, so much so that even some peptides degrade within 24 hours if you use the wrong reconstitution solution. The two most widely used solvents are:
Bacteriostatic water – is water that has been made to inhibit the growth of most types of bacteria. It is comprised of sterile and filtered water, with all bacteria removed, which is then mixed with 0.9% benzyl alcohol, which prevents any contaminating bacteria from growing in the water. In this way, the water has become ‘static’, or relatively unchanging in its bacterial content.
**Mostly all peptides are recommended to be reconstituted with this solvent with the exception of IGF peptides and Hydrophobic peptides (these peptides do not dissolve in water).
Acetic Acid – is a hydrophilic (polar) protic solvent, similar to ethanol and water. It dissolves not only polar compounds such as inorganic salts and sugars, but also non-polar compounds such as oils as well as polar solutes. It is miscible with polar and non-polar solvents such as water, chloroform, and hexane. Please note AA can damage muscle tissue if used by IM so it is recommend to “back-load” with sterile water or a small amount of Bacteriostatic water.
Peptides recommend for AA:
1. All IGF peptides: Using Bacteriostatic water with Insulin-like Growth Factor peptides can degrade and completely deteriorate it in as little as 24 hours due to the concentration of benzyl alcohol. (please advise: It is important to understand, you can not add bacteriostatic water nor sterile waters to your IGF-1 lr3, as it will not only mix with the already present acetic acid, like oil and water. The Benzyl Alcohol will degrade IGF-1 very fast! So unless its being used up in 1-2 days max, you should not add water to it!)
2. Hydrophobic peptides: such as HGH Fragment & Amyloid Beta. It is vitally important that before you begin researching with peptides, determine if it will be soluble in a solution that will be compatible with the experiments you wish to run. These be peptides will appear cloudy and not mix well.
Before adding any solvent to the lyophilized peptide, it is important to evaluate the amino acid composition of the peptide as a preliminary tool in understanding the solubility characteristics of your peptide.
PEPTIDE: IGF-1 LR3 (1mg)
DOSE/VIAL: 20 x 50mcgs
1. First, ALL of our peptides come VACUUM SEALED, so you will need to equalize the pressure inside the vial. Simply fill a syringe with air and insert it into the vial. The vacuum will draw the stopper to the end. Do this until this effect has stopped.
2. Swab the top of your IGF-1 vial with a sterile alcohol prep pad
3. Swab the top of your 0.6% AA vial with a sterile alcohol prep pad
4. f you have used 1ml of AA for mixing then a 50mcg dosage = 0.05ml (or 5 units on Insulin Syringe). Inject the ACETIC ACID very slowly and dribble it down the side of the vial. (be very careful with this peptide as it is very delicate!)
5. Remove the needle & syringe and discard
6. Gently swirl the vial or roll between your hands. Again, be very gentle here
7. You now have 1mg/mL of IGF-1 (This is the same as: 1,000mcg/mL)
8. You are now ready to apply these methods to your research.
1. Swab the top of your IGF-1 vial with a sterile alcohol prep pad
2. Draw the dosage (e.g. 50mcgs) you wish to research with into the syringe and eliminate all bubbles and trapped air.
3. Then Back-load, which is diluting the IGF-1/AA solution that is in your syringe. The point is to dilute the acidity to a point that it will no longer cause tissue necrosis (death/damage) or pain upon injection. It is recommended to dilute no less than 4:1 ratio (4 parts BW to 1 part IGF-1/AA)
4. EXAMPLE: IGF-1 LR3 : 2IUs of IGF-1 in syringe then add 8IUs of BAC